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polyfect transfection reagent  (Qiagen)


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    Structured Review

    Qiagen polyfect transfection reagent
    Polyfect Transfection Reagent, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 2436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyfect+reagent/PolyFect+Transfection+Reagent/pm42046515-109-52-55
    Average 97 stars, based on 2436 article reviews
    polyfect transfection reagent - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Transfection:

    Article Title: Epilepsy-Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties.
    Article Snippet: .. HEK293T cells (CRL- 3216; American Type Culture Collection, Manassas, VA) were transiently transfected with 2 μg WT or mutant NaV1.1 using PolyFect reagent (Qiagen, Germantown, MD). ..

    Article Title: Epilepsy-associated Variants of a Single SCN1A Codon exhibit Divergent Functional Properties
    Article Snippet: .. HEK293T cells (CRL-3216; American Type Culture Collection, Manassas, VA) were transiently transfected with 2 μg WT or mutant Na V 1.1 using PolyFect reagent (Qiagen, Germantown, MD). ..

    Article Title: miR-221 Exerts Antiviral and Anti-Inflammatory Effects against HSV-1 Through Direct Repression of CAMK2A and Immune Pathway Activation
    Article Snippet: .. Transfections were performed using Polyfect reagent (Qiagen, 301107, USA) following the manufacturer’s instructions for a 24-hour transfection. ..

    Article Title: Temperature and WNK-SPAK/OSR1 Kinases Dynamically Regulate Antiviral Human GFP-MxA Biomolecular Condensates in Oral Cancer Cells.
    Article Snippet: .. Transient transfections were carried out using the Polyfect reagent (Qiagen, Germantown, MD, USA) and the manufacturer’s protocol [23,48,49]. .. Live-cell imaging of GFP-MxA structures in transiently transfected cells was carried out in cells grown in 35 mm plates using the upright the Zeiss AxioImager 2 equipped with a temperature-regulated stage (range: 5–50 ◦C) and a 40× water immersion objective with data capture in a manual time-lapse mode (using Axiovision 4.8.1 software) [23,48,49].

    Article Title: TRPV6 channel function is involved in endometrial epithelial cell Ca 2+ signaling and female mouse fecundity
    Article Snippet: HEK-293 cells (ATCC, CRL 1573) and COS-7 cells (ATCC, CRL-1651), obtained from the American Type Culture Collection (ATCC, Manassas, VA), were cultured in 75 cm 2 flasks in minimal essential medium (MEM HEK-293) and Dulbecco’s modified eagles medium (DMEM COS-7) (both Life Technologies, Carlsbad, USA) containing 10% fetal calf serum (FCS; Life Technologies) at 37 °C and 5% CO 2 . .. For transient transfection, cells were grown in 3 cm diameter culture dishes until 80% confluence and then transiently transfected with 4 μg of pCAGGS-mTRPV6-IRES-GFP cDNA in 5 μL of the PolyFect ® reagent (Qiagen, Hilden, Germany). ..

    Article Title: Polynucleotides encoding APOA-1 fusion polypeptides
    Article Snippet: .. Clones with the correct sequence were then amplified and plasmid DNA was used for transient transfection of HEK293T cells using Polyfect reagent (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. Transfection media was changed after 24 hours to a low fluorescence, phenol red free media, FLUOROBRITETM DMEM (Life Technologies, Grand Island, NY), containing growth supplements (glutamine, pyruvate, non-essential amino acids, and pen/strep), but no serum.

    Article Title: miR-221 Exerts Antiviral and Anti-Inflammatory Effects against HSV-1 Through Direct Repression of CAMK2A and Immune Pathway Activation.
    Article Snippet: .. 163 Transfections were performed using Polyfect reagent (Qiagen, 301107, USA) 164 following the manufacturer’s instructions for a 24-hour transfection. ..

    Article Title: TRPV6 channel function is involved in endometrial epithelial cell Ca 2+ signaling and female mouse fecundity.
    Article Snippet: HEK-293 and COS-7 cell culture and transfection HEK-293 cells (ATCC, CRL 1573) and COS-7 cells (ATCC, CRL-1651), obtained from the American Type Culture Collection (ATCC, Manassas, VA), were cultured in 75 cm2 flasks in minimal essential medium (MEM HEK-293) and Dulbecco’s modified eagles medium (DMEM COS-7) (both Life Technologies, Carlsbad, USA) containing 10% fetal calf serum (FCS; Life Technologies) at 37 °C and 5% CO2. .. For transient transfection, cells were grown in 3 cm diameter culture dishes until 80% confluence and then transiently transfected with 4 μg of pCAGGS-mTRPV6IRES-GFP cDNA in 5 μL of the PolyFect® reagent (Qiagen, Hilden, Germany). ..

    Mutagenesis:

    Article Title: Epilepsy-Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties.
    Article Snippet: .. HEK293T cells (CRL- 3216; American Type Culture Collection, Manassas, VA) were transiently transfected with 2 μg WT or mutant NaV1.1 using PolyFect reagent (Qiagen, Germantown, MD). ..

    Article Title: Epilepsy-associated Variants of a Single SCN1A Codon exhibit Divergent Functional Properties
    Article Snippet: .. HEK293T cells (CRL-3216; American Type Culture Collection, Manassas, VA) were transiently transfected with 2 μg WT or mutant Na V 1.1 using PolyFect reagent (Qiagen, Germantown, MD). ..

    Clone Assay:

    Article Title: Polynucleotides encoding APOA-1 fusion polypeptides
    Article Snippet: .. Clones with the correct sequence were then amplified and plasmid DNA was used for transient transfection of HEK293T cells using Polyfect reagent (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. Transfection media was changed after 24 hours to a low fluorescence, phenol red free media, FLUOROBRITETM DMEM (Life Technologies, Grand Island, NY), containing growth supplements (glutamine, pyruvate, non-essential amino acids, and pen/strep), but no serum.

    Sequencing:

    Article Title: Polynucleotides encoding APOA-1 fusion polypeptides
    Article Snippet: .. Clones with the correct sequence were then amplified and plasmid DNA was used for transient transfection of HEK293T cells using Polyfect reagent (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. Transfection media was changed after 24 hours to a low fluorescence, phenol red free media, FLUOROBRITETM DMEM (Life Technologies, Grand Island, NY), containing growth supplements (glutamine, pyruvate, non-essential amino acids, and pen/strep), but no serum.

    Amplification:

    Article Title: Polynucleotides encoding APOA-1 fusion polypeptides
    Article Snippet: .. Clones with the correct sequence were then amplified and plasmid DNA was used for transient transfection of HEK293T cells using Polyfect reagent (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. Transfection media was changed after 24 hours to a low fluorescence, phenol red free media, FLUOROBRITETM DMEM (Life Technologies, Grand Island, NY), containing growth supplements (glutamine, pyruvate, non-essential amino acids, and pen/strep), but no serum.

    Plasmid Preparation:

    Article Title: Polynucleotides encoding APOA-1 fusion polypeptides
    Article Snippet: .. Clones with the correct sequence were then amplified and plasmid DNA was used for transient transfection of HEK293T cells using Polyfect reagent (QIAGEN, Valencia, CA), according to manufacturer's instructions. .. Transfection media was changed after 24 hours to a low fluorescence, phenol red free media, FLUOROBRITETM DMEM (Life Technologies, Grand Island, NY), containing growth supplements (glutamine, pyruvate, non-essential amino acids, and pen/strep), but no serum.



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